Cryo-EM structure of the mechanically activated ion channel OSCA1.2

Cryo-EM structure of the mechanically activated ion channel OSCA1.2

Blog Article

Mechanically activated ion channels underlie touch, spoon hearing, shear-stress sensing, and response to turgor pressure.OSCA/TMEM63s are a newly-identified family of eukaryotic mechanically activated ion channels opened by membrane tension.The structural underpinnings of OSCA/TMEM63 function are not explored.Here, we elucidate high resolution cryo-electron microscopy structures of OSCA1.2, revealing a dimeric architecture containing eleven transmembrane helices per subunit and surprising topological similarities to TMEM16 proteins.

We locate the ion permeation pathway within each subunit by demonstrating ULTIMATE MULTI PROBIOTIC that a conserved acidic residue is a determinant of channel conductance.Molecular dynamics simulations reveal membrane interactions, suggesting the role of lipids in OSCA1.2 gating.These results lay a foundation to decipher how the structural organization of OSCA/TMEM63 is suited for their roles as MA ion channels.